Targeted Clinical Metabolite Profiling Platform for the Stratification of Diabetic Patients [Elektronisk resurs]
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Ahonen, Linda (författare)
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Jäntti, Sirkku (författare)
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Suvitaival, Tommi (författare)
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Theilade, Simone (författare)
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Risz, Claudia (författare)
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Kostiainen, Risto (författare)
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Rossing, Peter (författare)
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Oresic, Matej, 1967- (författare)
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Hyötyläinen, Tuulia, 1971- (författare)
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Örebro universitet Institutionen för medicinska vetenskaper (utgivare)
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Örebro universitet Institutionen för naturvetenskap och teknik (utgivare)
- Publicerad: MDPI, 2019
- Engelska.
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Ingår i: Metabolites. - 2218-1989. ; 9:9
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- Relaterad länk:
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http://www.oru.se/ (Värdpublikation)
Sammanfattning
Ämnesord
Stäng
- Several small molecule biomarkers have been reported in the literature for prediction and diagnosis of (pre)diabetes, its co-morbidities, and complications. Here, we report the development and validation of a novel, quantitative method for the determination of a selected panel of 34 metabolite biomarkers from human plasma. We selected a panel of metabolites indicative of various clinically-relevant pathogenic stages of diabetes. We combined these candidate biomarkers into a single ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method and optimized it, prioritizing simplicity of sample preparation and time needed for analysis, enabling high-throughput analysis in clinical laboratory settings. We validated the method in terms of limits of detection (LOD) and quantitation (LOQ), linearity ( R 2 ), and intra- and inter-day repeatability of each metabolite. The method's performance was demonstrated in the analysis of selected samples from a diabetes cohort study. Metabolite levels were associated with clinical measurements and kidney complications in type 1 diabetes (T1D) patients. Specifically, both amino acids and amino acid-related analytes, as well as specific bile acids, were associated with macro-albuminuria. Additionally, specific bile acids were associated with glycemic control, anti-hypertensive medication, statin medication, and clinical lipid measurements. The developed analytical method is suitable for robust determination of selected plasma metabolites in the diabetes clinic.
Ämnesord
- Medical and Health Sciences (hsv)
- Medical Biotechnology (hsv)
- Other Medical Biotechnology (hsv)
- Medicin och hälsovetenskap (hsv)
- Medicinsk bioteknologi (hsv)
- Annan medicinsk bioteknologi (hsv)
Genre
- government publication (marcgt)
Indexterm och SAB-rubrik
- Clinical diagnostics
- diabetes
- mass spectrometry
- metabolomics
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