Single-Molecule Science : from super-resolution microscopy to DNA mapping and diagnostics / edited by Krishnarao Appasani, Raghu Kiran Appasani.

• PART I. Super-Resolution Microscopy and Molecular Imaging Techniques to Probe Biology -- 1. Introduction on Single-Molecule Science / Krishnarao Appasani, Raghu K. Appasani -- 2. One Molecule, Two Molecules, Red Molecules, Blue Molecules : Methods for Quantifying Localization Microscopy Data / Gaetan G. Herbomel, George H. Patterson -- 3. Multiscale Fluorescence Imaging / Manuel Gunkel, Jan Philipp Eberle, Ruben Bulkescher, Jürgen Reymann, Inn Chung, Ronald Simon, Guido Sauter, Vytaute Starkuviene, Karsten Rippe, Holger Erfle -- 4. Long-Read Single-Molecule Optical Maps / Assaf Grunwald, Yael Michaeli, Yuval Ebenstein -- PART II. Protein Folding, Structure, Confirmation, and Dynamics -- 5. Single-Molecule Mechanics of Protein Nanomachines / Gabriel Žoldák, Katarzyna Tych -- 6. Posttranslational Protein Translocation through Membranes at the Single-Molecule Level / Diego Quiroga-Roger, Hilda M. Alfaro-Valdés, Christian A. M. Wilson -- PART III. Mapping DNA Molecules at the Single-Molecule Level -- 7. Observing Dynamic States of Single-Molecule DNA and Proteins Using Atomic Force Microscope / Jingqiang Li, Sithara Wijeratne, Ching-Hwa Kiang -- 8. Atomic Force Microscopy and Detecting a DNA Biomarker of a Few Copies without Amplification / Sourav Mishra, Yoonhee Lee, Joon Won Park -- PART IV. Single-Molecule Biology to Study Gene Expression -- 9. Single-Molecule Detection in the Study of Gene Expression / Vipin Kumar, Simon Leclerc, Yuichi Taniguchi. 

• Single Molecule Science (SMS) has emerged from developing, using and combining technologies such as super-resolution microscopy, atomic force microscopy, and optical and magnetic tweezers, alongside sophisticated computational and modelling techniques. This comprehensive, edited volume brings together authoritative overviews of these methods from a biological perspective, and highlights how they can be used to observe and track individual molecules and monitor molecular interactions in living cells. Pioneers in this fast-moving field cover topics such as single molecule optical maps, nanomachines, and protein folding and dynamics. A particular emphasis is also given to mapping DNA molecules for diagnostic purposes, and the study of gene expression. With numerous illustrations, this book reveals how SMS has presented us with a new way of understanding life processes. A must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, biological imaging, genomics and structural biology. 

Ämnesord

Molekyler  (sao)

Molecules  (LCSH)

Molecules  (LCSH)

Indexterm och SAB-rubrik

Ucdc Atomfysik och molekylfysik

Klassifikation

539.6 (DDC)

Ucdc (kssb/8)