Arabidopsis thaliana plants expressing Rift Valley fever virus antigens [Elektronisk resurs] Mice exhibit systemic immune responses as the result of oraladministration of the transgenic plants
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Kalbina, Irina 1961- (författare)
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Lagerqvist, Nina (författare)
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Moiane, Bélisario (författare)
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Ahlm, Clas (författare)
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Andersson, Sören 1957- (författare)
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Strid, Åke 1960- (författare)
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Falk, Kerstin I. (författare)
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Örebro universitet Institutionen för naturvetenskap och teknik (utgivare)
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Örebro universitet Institutionen för medicinska vetenskaper (utgivare)
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Biokemi (medarbetare)
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Biokemi (medarbetare)
- San Diego, USA Elsevier 2016
- Engelska.
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Ingår i: Protein Expression and Purification. - 1046-5928. ; 127, 61-67
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Sammanfattning
Ämnesord
Stäng
- The zoonotic Rift Valley fever virus affects livestock and humans in Africa and on the Arabian Peninsula.The economic impact of this pathogen due to livestock losses, as well as its relevance to public health,underscores the importance of developing effective and easily distributed vaccines. Vaccines that can bedelivered orally are of particular interest. Here, we report the expression in transformed plants (Arabidopsis thaliana) of Rift Valley fever virusantigens. The antigens used in this study were the N protein and a deletion mutant of the Gn glycoprotein.Transformed lines were analysed for specific mRNA and protein content by RT-PCR and Westernblotting, respectively. Furthermore, the plant-expressed antigens were evaluated for their immunogenicityin mice fed the transgenic plants. After oral intake of fresh transgenic plant material, a proportionof the mice elicited specific IgG antibody responses, as compared to the control animals that were fedwild-type plants and of which none sero-converted. Thus, we show that transgenic plants can be readily used to express and produce Rift Valley Fever virusproteins, and that the plants are immunogenic when given orally to mice. These are promising findingsand provide a basis for further studies on edible plant vaccines against the Rift Valley fever virus.
Ämnesord
- Medical and Health Sciences (hsv)
- Basic Medicine (hsv)
- Immunology in the medical area (hsv)
- Medicin och hälsovetenskap (hsv)
- Medicinska grundvetenskaper (hsv)
- Immunologi inom det medicinska området (hsv)
- Natural Sciences (hsv)
- Biological Sciences (hsv)
- Immunology (hsv)
- Naturvetenskap (hsv)
- Biologiska vetenskaper (hsv)
- Immunologi (hsv)
- Biochemistry and Molecular Biology (hsv)
- Biokemi och molekylärbiologi (hsv)
- Medical Biotechnology (hsv)
- Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) (hsv)
- Medicinsk bioteknologi (hsv)
- Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci) (hsv)
- Biochemistry (oru)
- Biokemi (oru)
- Immunologi (oru)
- Immunology (oru)
Indexterm och SAB-rubrik
- Antigen production
- Arabidopsis thaliana
- Rift valley fever virus
- Plant vaccine
- Transformation
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