Class-A penicillin binding proteins do not contribute to cell shape but repair cell-wall defects [Elektronisk resurs]
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Vigouroux, Antoine (författare)
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Cordier, Baptiste (författare)
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Aristov, Andrey (författare)
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Alvarez, Laura (författare)
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Ozbaykal, Gizem (författare)
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Chaze, Thibault (författare)
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Oldewurtel, Enno Rainer (författare)
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Matondo, Mariette (författare)
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Cava, Felipe (författare)
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Bikard, David (författare)
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van Teeffelen, Sven (författare)
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Umeå universitet Medicinska fakulteten (utgivare)
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Umeå universitet Medicinska fakulteten (utgivare)
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Umeå universitet Medicinska fakulteten (utgivare)
- Publicerad: ELIFE SCIENCES PUBLICATIONS LTD, 2020
- Engelska.
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Ingår i: eLIFE. ; 9
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- Relaterad länk:
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http://www.umu.se/ (Värdpublikation)
Sammanfattning
Ämnesord
Stäng
- Cell shape and cell-envelope integrity of bacteria are determined by the peptidoglycan cell wall. In rod-shaped Escherichia coli, two conserved sets of machinery are essential for cell-wall insertion in the cylindrical part of the cell: the Rod complex and the class-A penicillin-binding proteins (aPBPs). While the Rod complex governs rod-like cell shape, aPBP function is less well understood. aPBPs were previously hypothesized to either work in concert with the Rod complex or to independently repair cell-wall defects. First, we demonstrate through modulation of enzyme levels that aPBPs do not contribute to rod-like cell shape but are required for mechanical stability, supporting their independent activity. By combining measurements of cell-wall stiffness, cell-wall insertion, and PBP1b motion at the single-molecule level, we then present evidence that PBP1b, the major aPBP, contributes to cell-wall integrity by repairing cell wall defects.
Ämnesord
- Medical and Health Sciences (hsv)
- Medical Biotechnology (hsv)
- Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) (hsv)
- Medicin och hälsovetenskap (hsv)
- Medicinsk bioteknologi (hsv)
- Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci) (hsv)
Genre
- government publication (marcgt)
Indexterm och SAB-rubrik
- CRISPRi
- E. coli
- cell biology
- cell envelope
- cell-wall repair
- infectious disease
- microbiology
- penicillin-binding proteins
- peptidoglycan cell wall
- single-molecule tracking
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