Transient Expression of Flavivirus Structural Proteins in Nicotiana benthamiana [Elektronisk resurs]
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Asghar, Naveed, 1983- (författare)
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Melik, Wessam, 1973- (författare)
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Paulsen, Katrine M. (författare)
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Pedersen, Benedikte N. (författare)
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Bø-Granquist, Erik G. (författare)
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Vikse, Rose (författare)
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Stuen, Snorre (författare)
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Andersson, Sören, 1957- (författare)
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Strid, Åke, 1960- (författare)
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Andreassen, Åshild K. (författare)
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Johansson, Magnus, 1969- (författare)
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Inflammatory Response and Infection Susceptibility Centre (iRiSC) (medarbetare)
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Inflammatory Response and Infection Susceptibility Centre (iRiSC) (medarbetare)
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Biokemi (medarbetare)
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Inflammatory Response and Infection Susceptibility Centre (iRiSC) (medarbetare)
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Örebro universitet Institutionen för medicinska vetenskaper (utgivare)
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Örebro universitet Institutionen för naturvetenskap och teknik (utgivare)
- Publicerad: MDPI, 2022
- Engelska.
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Ingår i: Vaccines. ; 10:10
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- Relaterad länk:
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http://www.oru.se/ (Värdpublikation)
Sammanfattning
Ämnesord
Stäng
- Flaviviruses are a threat to public health and can cause major disease outbreaks. Tick-borne encephalitis (TBE) is caused by a flavivirus, and it is one of the most important causes of viral encephalitis in Europe and is on the rise in Sweden. As there is no antiviral treatment availa-ble, vaccination remains the best protective measure against TBE. Currently available TBE vaccines are based on formalin-inactivated virus produced in cell culture. These vaccines must be delivered by intramuscular injection, have a burdensome immunization schedule, and may exhibit vaccine failure in certain populations. This project aimed to develop an edible TBE vaccine to trigger a stronger immune response through oral delivery of viral antigens to mucosal surfaces. We demonstrated successful expression and post-translational processing of flavivirus structural pro-teins which then self-assembled to form virus-like particles in Nicotiana benthamiana. We performed oral toxicity tests in mice using various plant species as potential bioreactors and evaluated the immunogenicity of the resulting edible vaccine candidate. Mice immunized with the edible vaccine candidate did not survive challenge with TBE virus. Interestingly, immunization of female mice with a commercial TBE vaccine can protect their offspring against TBE virus infection.
Ämnesord
- Medical and Health Sciences (hsv)
- Medical Biotechnology (hsv)
- Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) (hsv)
- Medicin och hälsovetenskap (hsv)
- Medicinsk bioteknologi (hsv)
- Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci) (hsv)
- Basic Medicine (hsv)
- Microbiology in the medical area (hsv)
- Medicinska och farmaceutiska grundvetenskaper (hsv)
- Mikrobiologi inom det medicinska området (hsv)
- Immunology in the medical area (hsv)
- Immunologi inom det medicinska området (hsv)
- Natural Sciences (hsv)
- Biological Sciences (hsv)
- Biochemistry and Molecular Biology (hsv)
- Naturvetenskap (hsv)
- Biologiska vetenskaper (hsv)
- Biokemi och molekylärbiologi (hsv)
- Microbiology (oru)
- Mikrobiologi (oru)
- Biochemistry (oru)
- Biokemi (oru)
- Immunologi (oru)
- Immunology (oru)
- Biology (oru)
- Biologi (oru)
Genre
- government publication (marcgt)
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