Startsida
Hjälp
Sök i LIBRIS databas

     

 

Sökning: onr:12038787 > On microvascular bl...

On microvascular blood flow assessment with the new microanalysis urea clearance technique / Simon Farnebo.

Farnebo, Simon, 1972- (författare)
ISBN 9789173933209
Publicerad: Linköping : Department of Clinical and Experimental Medicine, Linköping University, 2010
Engelska 69 sidor
Serie: Linköping University medical dissertations, 0345-0082 ; 1204
  • BokAvhandling(Diss. (sammanfattning) Linköping : Linköpings universitet, 2010)
Sammanfattning Ämnesord
Stäng  
  • The aim of this thesis was to develop and evaluate a new way of monitoring blood flow with microdialysis. A thin catheter consisting of a semipermeable membrane is implanted in the tissue being studied. The catheter is perfused by a solution that closely resembles interstitial fluid, and small water-soluble substances are allowed to diffuse passively through the pores of the membrane with the aim at reaching equilibrium with the surrounding tissue. The minimally invasive character of microdialysis, and its ability to sample from the organ being studied, make microdialysis attractive in most research settings as well as for clinical surveillance. It has, however, become increasingly evident that microdialysis under conditions of non-equilibrium - for example, fluctuating regional blood flow, will alter the results gained. We have therefore aimed to explore the possibilities of developing a new marker of blood flow that will yield information about changes in blood flow that occur in the area of the microdialysis catheter itself. We hypothesised that the changes in the diffusion of exogenous urea could be used as markers of changes in tissue blood flow. The theoretical basis for this approach is that the mass transfer of urea will increase across the dialysis membrane secondary to increased blood flow. As removal of urea from the vicinity of the dialysis membrane increases with increased blood flow, the concentration gradient of urea between the perfusate and tissue will also increase. This in turn will result in a greater loss of urea from the perfusate. The changes noted in retrieval of urea from dialysate by the system are therefore thought to be inversely related to changes in blood flow. We tested our hypothesis in two species of animal (rat and pig) and in man, and in three organ systems (muscle, liver, and skin), and present four papers that indicate that the urea clearance technique provides reliable and reproducible results. The technique was evaluated against conventional metabolic markers (lactate and glucose), the ethanol clearance technique (microdialysis), laser Doppler perfusion imaging (LDPI), and polarisation light spectroscopy (TiVi). 
  • We present evidence that the urea clearance technique can be used to assess blood flow in the organs studied reliably and reproducibly with microdialysis. The microdialysis technique is minimally invasive and safe for the recipient, and catheters can easily be implanted during operation to monitor organs at risk. Urea is easily analysed as a standard assay among other “basic” metabolic markers (in a standard microdialysis kit) and has favourable characteristics with a standardised measurement system that is routinely used for monitoring metabolites in the clinic. The technique is also effective when used at lower perfusate flow rates (<1 μl/minute), which is advantageous as the recovery of metabolic markers increases at low perfusate flow rates. 

Ämnesord

Microdialysis  -- methods (MeSH)
Muscle, skeletal  -- blood supply (MeSH)
Muscle, skeletal  -- metabolism (MeSH)
Regional blood flow  -- physiology (MeSH)
Urea  -- blood (MeSH)

Indexterm och SAB-rubrik

Ved Blodet och de blodbildande organen (hematologi)

Klassifikation

616.4 (DDC)
Ved (kssb/8)
Inställningar Hjälp

Titeln finns på 4 bibliotek. 

Bibliotek i norra Sverige (1)

Ange som favorit

Bibliotek i Stockholmsregionen (1)

Ange som favorit

Bibliotek i östra Sverige (1)

Ange som favorit

Bibliotek i södra Sverige (1)

Ange som favorit

Sök vidare

Hjälp
Fler titlar av
Farnebo, Simon, 1972 ...
Fler titlar om
Microdialysis
Microdialysis
och methods
Muscle, skeletal
Muscle, skeletal
och blood supply
Muscle, skeletal
Muscle, skeletal
och metabolism
Regional blood flow
Regional blood flow
och physiology
Urea
Urea
och blood
Serie
Fler delar
Andra versioner
On microvascular blo ...

Sök utanför LIBRIS

Hjälp
Om LIBRIS
Sekretess
Hjälp
Fel i posten?
Kontakt
Teknik och format
Sök utifrån
Sökrutor
Plug-ins
Bookmarklet
Anpassa
Textstorlek
Kontrast
Vyer
LIBRIS söktjänster
SwePub
Uppsök

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

Copyright © LIBRIS - Nationella bibliotekssystem

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy