Readout Strategies for Biomolecular Analyses [Elektronisk resurs]
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Göransson, Jenny, 1978- (författare)
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Nilsson, Mats (preses)
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Cantor, Charles (opponent)
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Uppsala universitet Medicinska och farmaceutiska vetenskapsområdet (utgivare)
- Publicerad: Uppsala : Acta Universitatis Upsaliensis, 2008
- Engelska 41
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Serie: Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, 1651-6214 1651-6214 ; 563
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Sammanfattning
Ämnesord
Stäng
- This thesis describes three readout formats for molecular analyses. A common feature in all works is probing techniques that upon specific target recognition ideally results in equimolar amounts of DNA circles. These are then specifically amplified and detected using any of the techniques presented herein. The first paper presents a method that enables homogeneous digital detection and enumeration of biomolecules, represented as fluorescence-labelled DNA macromolecules. This method offers precise measurements to be performed with a wide linear dynamic range. As an application, two closely related bacterial species were selectively detected. The second paper further investigates and optimizes the properties of the technique presented in paper one. The third paper demonstrates a platform that enables simultaneous quantitative analysis of large numbers of biomolecules. The array format and decoding scheme together propose a digital strategy for decoding of biomolecules. The array and the decoding procedure were characterized and evaluated for gene copy-number measurements. The fourth paper examines a new strategy for non-optical measurements of biomolecules. Characteristics of this technique are investigated, and compared to its optical equivalent, fluorescence polarization.
Ämnesord
- Medical and Health Sciences (hsv)
- Basic Medicine (hsv)
- Medical Genetics (hsv)
- Medicin och hälsovetenskap (hsv)
- Medicinska och farmaceutiska grundvetenskaper (hsv)
- Medicinsk genetik (hsv)
- MEDICINE (svep)
- Dermatology and venerology,clinical genetics, internal medicine (svep)
- Internal medicine (svep)
- Molecular medicine (genetics and pathology) (svep)
- MEDICIN (svep)
- Dermatologi och venerologi, klinisk genetik, invärtesmedicin (svep)
- Invärtesmedicin (svep)
- Molekylär medicin (genetik och patologi) (svep)
Genre
- government publication (marcgt)
Indexterm och SAB-rubrik
- Padlock probe
- Selector probe
- Rolling circle amplification
- Single molecule detection
- Amplified single molecule
- Random array
Inställningar
Hjälp
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